Cell culture medium Assignment | College Homework Help

Q1. Why is it important to have Ca2+ free conditions when passaging cells? O Ca2+ binds to and impairs the activity of trypsin. O Ca2+ promotes cell-cell and cell-matrix adhesion, making cells more difficult to trypsinise. O Ca2+ interferes with Trypan Blue staining, rendering cell counts inaccurate. O Ca2+ will react with the phosphate in PBS to form a solid, making it difficult to pipette cells. Q2. Which of the following culture medium ingredients is required for cell proliferation in vitro? serum lactic acid Phenol red ammonium Q3. What determines the time interval for replacement of cell culture medium? the growth rate of the cells the type of culture vessel used the presence of serum the evaporation of medium from the vessel Q4. Aseptic technique involves: O pouring media and reagents directly into culture flasks removing the caps from culture flasks and tubes immediately before use putting culture flasks and tubes near the opening of the biosafety cabinet O Frequent cleaning of work surfaces and hands with water Q5. Which of the following series of events depicts the correct order for cell passaging? 1 Aspirate cells from culture vessel, count cell number using haemocytometer, place cells into new vessel Remove medium from culture vessel, wash with PBS, add new medium containing serum O Incubate cells with trypsin, remove cells, centrifuge and resuspend in medium containing serum, dilute cells and place into new vessel O Remove culture medium, wash cells with trypsin, incubate with EDTA-containing medium, aspirate cells and place into new vessel at lower concentration Q6. During cell counting live cells can be distinguished from dead cells by: 1 O The absence of trypan blue staining The irregular shape of live cells O The presence of cell processes O The presence of tight junctions Q7. Why is it necessary to perform cell dissociation in the absence of serum? 1 pt serum contains Ca2+ 0 0 O serum chelates divalent metal ions O serum inhibits trypsin O serum stimulates cell proliferation 1 pt O Q8. Using the following image calculate the concentration of cells in a 1 mL sample from which 10 uL of cell suspension was added to 90 uL of trypan blue. The haemocytometer used had a volume of 100 nL per large square. OO O 4.25 x 104 b o O 4.75 104 O 10 0 4.25 x 105 O 4.75 x 105 Q9. How does Trypan Blue dye enter cells during cell counting? 1 pt Enter your answer here MORE + Q10. A student forgets to add trypsin when passaging cells. What would be the result of this? O The media will become acidic and the cells will die The cells will not lift off the plate during passaging and so the cell count will be low O The cells will not have sufficient growth factors and so proliferation will be limited The cells will clump, making it difficult to count the cells on the haemocytometer Get Health Science homework help today
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